Đã xuất bản: 12/11/2025
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Quang Huy Vu - Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes - 2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035568/
Establishment of an Experimental Procedure for Preparing Trial Serum Samples for the Specific Serodiagnosis of Toxocara canis for External Quality Assessment Schemes
Quang Huy Vu, Diep Tuan Tran, Phu Manh Sieu Tran, Van Chuong Le, Thi Diem Phuc Huynh, and Quang Sang Bui
Tóm tắt
Background
External quality assessment (EQA) provides evidence of
reliable, accurate, and precise results for customers using the diagnostic test
for Toxocara canis. Objective. To establish a procedure for producing standard
Toxocara canis serum samples for serodiagnostic testing in EQA.
Methods
The collected serum samples to contain anti-Toxocara canis
serum samples for serodiagnostic testing in EQA. F-test and a T-test were
applied to evaluate their homogeneity and stability.
Results
Among eleven samples positive by ELISA, ten of them were
confirmed via Western blotting by positive reaction with 5 specific Toxocara
canis serum samples for serodiagnostic testing in EQA. Toxocara canis serum
samples for serodiagnostic testing in EQA. p > 0.05). Samples produced by
both methods were stable for 7 days at 30°C (p > 0.05). Samples produced by
both methods were stable for 7 days at 30°C (
Conclusion
Specific serodiagnosis samples of anti-Toxocara canis
antibodies for EQA could be produced that possessed homogeneity and stability
lasting for 3 months and 6 months by the freeze-drying and freezing methods,
respectively. At 30°C, the samples produced by both methods were stable for 7
days, suitable for delivery to remote laboratories.Toxocara canis serum samples
for serodiagnostic testing in EQA.
